selective jak2 inhibitor ag490  (Merck KGaA)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Exercise‐induced peptide EIP‐22 protect myocardial from ischaemia/reperfusion injury via activating JAK2/STAT3 signalling pathway

doi: 10.1111/jcmm.16441

Figure Lengend Snippet: EIP‐22 activates JAK2/STAT3 signalling pathway. A, The RNA‐seq approach was used to map the transcriptome after EIP‐22 treatment in H/R model. The dysregulated genes were shown in the volcano plot, P ‐value < .05 and fold change >2. B, Heat map of the differentially expressed genes. Red indicates up‐regulation, and green indicates down‐regulation. C, KEGG pathway analysis. D, The main proteins involved in JAK2/STAT3 signalling pathway were analysed by Western blot in H/R model. E, Quantification data of Western blot analysis. F, The main proteins involved in JAK2/STAT3 signalling pathway were analysed by Western blot in H 2 O 2 model. G, Quantification data of Western blot analysis. The data represent means ± SD. ** P < .01 vs. the Scr group, *** P < .001 vs. the Scr group, # P < .05 vs. the H/R + Scr or H 2 O 2 + Scr group, ## P < .01 vs. the H/R + Scr or H 2 O 2 + Scr group

Article Snippet: The AG490, a selective inhibitor of JAK2/STAT3, was purchased from Sigma Chemical Co.

Techniques: RNA Sequencing Assay, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Exercise‐induced peptide EIP‐22 protect myocardial from ischaemia/reperfusion injury via activating JAK2/STAT3 signalling pathway

doi: 10.1111/jcmm.16441

Figure Lengend Snippet: AG490 eliminates the cardioprotection of EIP‐22. A, The protein levels of p‐JAK2 and p‐STAT3 were inhibited whereas cells were treated with AG490 in H/R model. B, Quantification data of expression level of p‐JAK2 and p‐STAT3 in H/R model. C, The protein levels of p‐JAK2 and p‐STAT3 were inhibited whereas cells were treated with AG490 in H 2 O 2 model. D, Quantification data of expression level of p‐JAK2 and p‐STAT3 in H 2 O 2 model. E, The detection of cell viability in H/R model. F, The detection of cell viability in H 2 O 2 model. G, The detection of LDH level in H/R model. H, The detection of LDH level in H 2 O 2 model. I, The proteins related to apoptosis were detected in H/R model by Western blot analysis. J, Quantification data of Western blot analysis. K, The proteins related to apoptosis were detected in H 2 O 2 model by Western blot analysis. L, Quantification data of Western blot analysis. The data represent means ± SD. ** P < .01 vs. the Scr group, *** P < .001 vs. the Scr group, # P < .05 vs. the H/R + Scr or H 2 O 2 + Scr group, ## P < .01 vs. the H/R + Scr or H 2 O 2 + Scr group, & P < .05 vs. the H/R + EIP‐22 or H 2 O 2 + EIP‐22 group, && P < .01 vs. the H/R + EIP‐22 or H 2 O 2 + EIP‐22 group

Article Snippet: The AG490, a selective inhibitor of JAK2/STAT3, was purchased from Sigma Chemical Co.

Techniques: Expressing, Western Blot

Journal: JAK-STAT

Article Title: Propofol mediates signal transducer and activator of transcription 3 activation and crosstalk with phosphoinositide 3-kinase/AKT

doi: 10.4161/jkst.29554

Figure Lengend Snippet: Figure 2. Effects of PI3K/AKT or JAK2/STAT3 signaling inhibition on propofol-induced STAT3 and AKT phosphorylation. Cells were pre-incubated with different inhibitors for 30 min, and then treated with propofol for another 30 min. Cell lysates were collected and AKT and STAT3 phosphorylation was detected. ( A ) Pretreatment with wortmannin (Wort) or API-2 reduced STAT3 phosphorylation at tyr705. ( B ) Wortmannin or API-2 pretreatment reduced propofol-induced STAT3 phosphorylation at ser727. ( C and D ) Pretreatment with AG490 or stattic inhibited propofol-induced AKT phosphorylation at ser473 and thr308. No changes in protein expression were observed with total STAT3 and total AKT in all groups ( A–D ).

Article Snippet: AG490 (selective JAK2 inhibitor) was purchased from Cayman Chemical.

Techniques: Inhibition, Incubation, Expressing